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Product Info Summary
| SKU: | M00780 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-LAMP1/Cd107A Rabbit Monoclonal Antibody
View all LAMP-1/CD107a Antibodies
SKU/Catalog Number
M00780
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-LAMP1/Cd107A Rabbit Monoclonal Antibody catalog # M00780. Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LAMP1/Cd107A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00780)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
IIB-12
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human LAMP1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00780 is reactive to LAMP1 in Human
Applications
M00780 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
15 kDa
Calculated molecular weight
44.882kDa
Background of LAMP-1/CD107a
Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:6K dilution for 1 hour at room temperature.
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Western blot analysis of LAMP1 expression in A431 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For LAMP1 (Source: Uniprot.org, NCBI)
Gene Name
LAMP1
Full Name
Lysosome-associated membrane glycoprotein 1
Weight
44.882kDa
Superfamily
LAMP family
Alternative Names
CD107 antigen-like family member A; CD107a antigen; CD107a; LAMP1; LAMP-1; LAMPA; LGP120; lysosomal-associated membrane protein 1; lysosome-associated membrane glycoprotein 1; Lysosome-associated membrane protein 1 LAMP1 CD107a, LAMPA, LGP120 lysosomal associated membrane protein 1 lysosome-associated membrane glycoprotein 1|CD107 antigen-like family member A|LAMP-1|lysosome-associated membrane protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LAMP1, check out the LAMP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LAMP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LAMP1/Cd107A Rabbit Monoclonal Antibody (M00780)
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No publications found for M00780
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-LAMP1/Cd107A Rabbit Monoclonal Antibody
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-LAMP1/Cd107A Rabbit Monoclonal antibody M00780. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-05-07
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-05-07
Question
We are currently using anti-LAMP1/Cd107A Rabbit Monoclonal antibody M00780 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
The anti-LAMP1/Cd107A Rabbit Monoclonal antibody (M00780) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-19
Question
I would like using your anti-LAMP1/Cd107A Rabbit Monoclonal antibody for neutrophil degranulation studies. Has this antibody been tested with western blotting on a431 cell lysate? We would like to see some validation images before ordering.
W. Mitchell
Verified customer
Asked: 2019-11-20
Answer
Thanks for your inquiry. This M00780 anti-LAMP1/Cd107A Rabbit Monoclonal antibody is tested on a431 cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-11-20
Question
Can you help my question with product M00780, anti-LAMP1/Cd107A Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-05
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00780 anti-LAMP1/Cd107A Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-05
Question
We have observed staining in human pancreas testis. Are there any suggestions? Is anti-LAMP1/Cd107A Rabbit Monoclonal antibody supposed to stain pancreas testis positively?
Verified Customer
Verified customer
Asked: 2019-08-08
Answer
From what I have seen in literature pancreas testis does express LAMP1. From what I have seen in Uniprot.org, LAMP1 is expressed in nephron tubule, mammary gland, brain, pancreas testis, placenta, plasma, liver, among other tissues. Regarding which tissues have LAMP1 expression, here are a few articles citing expression in various tissues:
Brain, Pancreas, and Testis, Pubmed ID: 15489334
Liver, Pubmed ID: 19159218, 24275569
Mammary gland, Pubmed ID: 14702039
Placenta, Pubmed ID: 17897319, 8517882, 2584229, 2243102, 8323299
Plasma, Pubmed ID: 16335952
Boster Scientific Support
Answered: 2019-08-08
Question
I see that the anti-LAMP1/Cd107A Rabbit Monoclonal antibody M00780 works with WB, what is the protocol used to produce the result images on the product page?
D. Jackson
Verified customer
Asked: 2019-07-18
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-18
Question
Is there a BSA free version of anti-LAMP1/Cd107A Rabbit Monoclonal antibody M00780 available?
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-LAMP1/Cd107A Rabbit Monoclonal antibody M00780 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-11
Question
Does M00780 anti-LAMP1/Cd107A Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
As indicated on the product datasheet, M00780 anti-LAMP1/Cd107A Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-24
Question
I was wanting to use your anti-LAMP1/Cd107A Rabbit Monoclonal antibody for WB for human brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human brain identification?
Verified Customer
Verified customer
Asked: 2018-11-19
Answer
It shows on the product datasheet, M00780 anti-LAMP1/Cd107A Rabbit Monoclonal antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-11-19
Question
Is a blocking peptide available for product anti-LAMP1/Cd107A Rabbit Monoclonal antibody (M00780)?
M. Singh
Verified customer
Asked: 2018-09-21
Answer
We do provide the blocking peptide for product anti-LAMP1/Cd107A Rabbit Monoclonal antibody (M00780). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-09-21
Question
you antibody to test anti-LAMP1/Cd107A Rabbit Monoclonal antibody M00780 on human brain for research purposes, then I may be interested in using anti-LAMP1/Cd107A Rabbit Monoclonal antibody M00780 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-01-19
Answer
The products we sell, including anti-LAMP1/Cd107A Rabbit Monoclonal antibody M00780, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-01-19
Question
Is this M00780 anti-LAMP1/Cd107A Rabbit Monoclonal antibody reactive to the isotypes of LAMP1?
K. Anderson
Verified customer
Asked: 2017-01-13
Answer
The immunogen of M00780 anti-LAMP1/Cd107A Rabbit Monoclonal antibody is A synthesized peptide derived from human LAMP1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-01-13
Question
Does anti-LAMP1/Cd107A Rabbit Monoclonal antibody M00780 work for WB with brain?
R. Parker
Verified customer
Asked: 2016-12-30
Answer
According to the expression profile of brain, LAMP1 is highly expressed in brain. So, it is likely that anti-LAMP1/Cd107A Rabbit Monoclonal antibody M00780 will work for WB with brain.
Boster Scientific Support
Answered: 2016-12-30
Question
Here is the WB image, lot number and protocol we used for brain using anti-LAMP1/Cd107A Rabbit Monoclonal antibody M00780. Please let me know if you require anything else.
C. Edwards
Verified customer
Asked: 2015-05-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-05-01
Question
Our lab were happy with the WB result of your anti-LAMP1/Cd107A Rabbit Monoclonal antibody. However we have been able to see positive staining in liver cell membrane using this antibody. Is that expected? Could you tell me where is LAMP1 supposed to be expressed?
A. Krishna
Verified customer
Asked: 2013-03-22
Answer
From literature, liver does express LAMP1. Generally LAMP1 expresses in cell membrane. Regarding which tissues have LAMP1 expression, here are a few articles citing expression in various tissues:
Brain, Pancreas, and Testis, Pubmed ID: 15489334
Liver, Pubmed ID: 19159218, 24275569
Mammary gland, Pubmed ID: 14702039
Placenta, Pubmed ID: 17897319, 8517882, 2584229, 2243102, 8323299
Plasma, Pubmed ID: 16335952
Boster Scientific Support
Answered: 2013-03-22
